2014-04-13 CRISPRdirect: designing CRISPR/Cas guide RNA sequence

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CRISPRdirectefficiently designs guide RNA (gRNA) against any input DNA sequence. CRISPR/Cas system is a simple genome editing technology that enables to cut genome at any desired location. Guide RNA used in this system must flank the PAM sequence and have high specificity to avoid off-target editing. You can also watch YouTube version of this togotv.

DOI: 10.7875/togotv.2014.026