統合TV 統合TVは、ライフサイエンス統合データベースセンター(DBCLS)が発信する生命科学分野の有用なデータベース(DB)やウェブツールの活用法を動画で紹介するウェブサイトです。くわしくは はじめての方へ をご覧ください。ご質問、ご要望は、お問い合わせ もしくは ライフサイエンスQA からお願いします。TogoTV beta ('togo' means 'integration' in Japanese) is an archive of tutorial videos expounding how to use biological databases and tools. Although most of contents are currently depicted in Japanese, you can find English contents in TogoTV international. Please post any comments, suggestions, and requests at each content page, e-mail to the address described at the bottom of each page or contact form. This site is provided by Database Center for Life Science (DBCLS) and funded by MEXT Integrated Database Project in Japan.

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TogoTV paper published in Briefings in Bioinformatics. (2011/7/29)

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2011-12-15

_ [presentation][English] ゲノムメチル化とエピジェネティクスIII:Genome Methylation and Epigenetics III: DNA Communications by Type III Restriction Enzymes: 1D looping or 3D looping or Both?

ゲノムメチル化とエピジェネティクスIII: DNA Communications by Type III Restriction Enzymes: 1D looping or 3D looping or Both?

本日の統合TVは、2011年度夏学期(4〜7月)に開講された、東京大学グローバルCOEプログラム「ゲノム情報ビッグバンから読み解く生命圏新領域創成科学特別講義IIから、Department of Biochemistry, Indian Institute of Science の Prof. Desirazu Narasimha Rao による「ゲノムメチル化とエピジェネティクスIII: DNA Communications by Type III Restriction Enzymes: 1D looping or 3D looping or Both?」をお送りします。

This lecture is talked by Prof. Desirazu Narasimha Rao from Department of Biochemistry, Indian Institute of Science entitled "Genome Methylation and Epigenetics III: DNA Communications by Type III Restriction Enzymes: 1D looping or 3D looping or Both?". It is a part of "Frontier Sciences Special Lecture Series II" in The University of Tokyo Global COE Program: Deciphering Biosphere from Genome Big Bang.

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Keywords: GCOE,Genome big bang,type III restriction enzyme

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DNA Communications by Type III Restriction Enzymes: 1D looping or 3D looping or Both? Types of Restriction-Modification Enzymes Type III Restriction-Modification Enzymes R.EcoP15I (Type III) Multiple turnover Functional co-operation with exonuclease Nucleic Acids Research cover Restriction requires two unmodified recognition sites that can be separated by different distances but which must be in inverse orientation Type III restriction endonucleases translocate DNA in a reaction driven by recognition site-specific ATP hydrolysis EcoP15I cleavage depends on the enzyme site ratio and is blocked by Lac repressor bound between sites Mechanistic model of DNA Restriction by ATP </p>
<p>dependent Restriction enzymes TRACKING - COLLISION MODEL SFM Images of EcoP15I-DNA complexes Inspection of Enlarged SFM Images of Single DNA Loops Blocking/Reduced Cooperation and DNA Cleavage Sequence context Cleavage of non head-to-head sites Interaction with DNA end on 5 of site Interaction with DNA end on 3 of site Protein translocation model Reversal of direction Nucleic Acids Research cover With Multiple pair substrates Lac repressor and Sinefungin DNA cleavage application Looping Diffusive Looping R.EcoP15 I EcoP15I–DNA complexes in the presence of ATP-gamma-S on one site template DNA EcoP15I-DNA complexes in the presence of ATP on one site template EcoP15I-DNA complexes in the presence of ATP on one site template EcoP15I - DNA complexes in the presence of ATP on two-site template Lac repressor blocks DNA cleavage by EcoP15I Comparison of loop contour length with a polymer physics model Consecutive time - lapse images of an EcoP15I-DNA complex obtained at 10 frames per s, using fast-scan AFM Translocation and extruded looping followed by diffusive looping by EcoP15I Search strategies used by restriction enzymes requiring two target sites for cleavage Structural model for DNA cleavage by EcoP15I Acknowledgments


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